Home Page

Isosurface reconstruction of an isolated Vorticella stalk. Data collected using a Zeiss LSM510-Meta confocal microscope, deconvolved using Huygens2 (Scientific Volume Imaging) and rendered using Imaris (Bitplane).
Valeriya Baru, Matsudaira Lab, Whitehead Institute

Wireframe representation of 40 µm resolution MRI data showing internal anatomy of an unanesthetized fly (Sarcophaga bullata). Imaging was performed on a 14.1 T MRI microscope using a spin echo pulse sequence.
Alan Jasanoff, Whitehead Institute and Whitehead-MIT BioImaging Center

Integrin colocalization with podosomes at the leading edge of an IC-21 macrophage. Various views before and after deconvolution. Data was collected using a Nikon widefield microscope and deconvolved using Huygens2 (Scientific Volume Imaging).
James G. Evans, CSBi & Whitehead-MIT BioImaging Center

Search/SiteMap/Contact

Time-sequence of β-actin-EYFP in podosomes at the leading edge of an IC-21 macrophage, imaged using a Zeiss LSM510-Meta confocal microscope and deconvolved using Huygens2 (Scientific Volume Imaging).
James G. Evans, CSBi & Whitehead-MIT BioImaging Center

About Us

Vimentin (red) and fimbrin (green) localization in an IC-21 macrophage. Fimbrin and vimentin complexes colocalize in podosomes at the leading edge and in retraction fibers at the trailing edge. Data collected using a Nikon epifluorescence microscope.
Ivan Correia, former member of the Matsudaira Lab, Whitehead Institute.

Research

Isosurface reconstruction (using Imaris, Bitplane) of a giant unilamellar vesicle (GUV) comprised of 20mol% cholesterol, 39.9 mol% DOPC, 39.9 mol% DPPC and 0.2mol% Texas Red®-DPPE. Data was collected using a Perkin Elmer UltraViewRS spinning-disk confocal microscope and deconvolved using Huygens2 (Scientific Volume Imaging).
Margaret Hornton, Gast Lab, MIT

Strategy

40 µm resolution MRI data showing internal anatomy of an unanesthetized fly (Sarcophaga bullata). Imaging was performed on a 14.1 T MRI microscope using a spin echo pulse sequence.
Alan Jasanoff, Whitehead Institute and Whitehead-MIT BioImaging Center

Services

Isosurface reconstruction of a podosome cluster precursor (PCP, green) and daughter podosomes (red) extracted from a 4D data set in which β-actin-EYFP was imaged in an IC-21 macrophage using a Zeiss LSM510-Meta confocal microscope. Data was deconvolved using Huygens2 (Scientific Volume Imaging) and rendered using Imaris (Bitplane).
James G. Evans, CSBi & Whitehead-MIT BioImaging Center

News & Events

Metaphase spread in Drosophila S2 cells, chromosomes stained with DAPI (blue) and centromere stained using anti-CID/CENP-A (Karpen Lab, Lawrence Berkeley National Lab and UC Berkeley) shown in green. Image data was collected on an Applied Precision Spectris widefield microscope and deconvolved using Huygens2 (Scientific Volume Imaging). Image shown is a maximum intensity projection.
Janice Lee, Orr-Weaver Lab, Whitehead Institute.