Measure | Cryoelectron Microscopy

At the BioImaging Center, image acquisition can be achieved through a number of microscopy methods, including (from lower to higher resolution) nuclear magnetic resonance (NMR) imaging; high-resolution, high-content, high-throughput light microscopy; high-resolution cryoelectron microscopy; and time-resolved atomic force microscopy (AFM). With these tools, we can capture images of cellular and molecular structures down to the nanometer level.

Sharpening the image

Often, processing is needed to sharpen the acquired image by removing artifacts from the microscope. In confocal microscopes, a pinhole removes out-of-focus photons, while an energy filter in an electron microscope eliminates out-of-focus electrons. One common computational method of processing involves deconvolution, which increases the resolution of light microscopy data and removes the noise and blur that often accompany high rates of data acquisition.